采用可行的技术调控土壤及作物根际微生物区系是恢复发枯萎病蕉园香蕉健康种植的前提，也是解决香蕉连作生物障碍的根本途径。课题组前期研究结果表明，秸秆淹水预处理高发枯萎病蕉园土壤然后轮作水稻可显著降低土壤中尖孢镰刀菌的数量,下茬香蕉土传枯萎病的发病率显著降低，然而，秸秆淹水预处理土壤联合水稻轮作杀灭土壤中病原菌机理及其对土壤中其它微生物的影响，以及种植下茬香蕉后，香蕉根际土壤微生物群落结构与根际病原微生物之间的关系需要进行更深入的研究。本项目拟利用MiSeq测序技术分析土壤微生物具体类群，辅以Real-Time PCR技术测定微生物数量，分析土壤、作物根际土壤微生物区系的变化，解析土壤根际微生物区系的机制，为高发枯萎病蕉园的恢复种植提供理论依据。

Manipulated soil and rhizophere microflora by acceptable method is the basement for healthy replanting abandoned areas caused by Fusarium oxysporum f. sp. cubense (FOC), and also the fundamental way to prevent banana soil-borne disease. Our preliminary experiments showed that flooding with straw combined with rice-rotation applied significantly reduced the number of Fusarium oxysporum in the abandoned soils and prevent next-season banana Fusarium wilt disease. However, the mechanism of the elimination of the pathogen and its impact other microorganisms in these soils by this method still unexplored, In addition, the relationship between rhizosphere microflora and pathogen also need further investigation. In this project, MiSeq sequencing technique will be used to analyze the specific microbial community and structure. Real-Time PCR technique will be used to detect the number of microbes. Then the changes and characteristics of the soil and rhizosphere microflora of banana plantations will be studied. This project will find out the mechanisms involved in rhizosphere microflora reconstruction from bulk soil and provide an important theoretical basis for replanting bananas in areas with serious continuous cropping obstacle.