双孢蘑菇是世界上栽培最广、产量最多、消费最普遍的一种食用菌，麦角甾醇作为双孢蘑菇中代表性的功能活性物质和维生素D原，其含量较低且国内外关于如何提高双孢蘑菇中麦角甾醇含量的研究未见报道，我们前期研究发现，茉莉酸甲酯（MeJA）处理能够显著增加双孢蘑菇中麦角甾醇的含量，但其诱导的分子机制仍不明确。本研究基于这一关键科学问题，按照“MeJA处理→诱导基因表达→调控蛋白表达→影响相关酶活→促进麦角甾醇积累”研究思路，分析MeJA处理对双孢蘑菇中麦角甾醇代谢规律的影响，并利用转录组学、蛋白组学、酶学等方法探讨MeJA处理在转录水平和蛋白表达水平以及合成酶活性水平三个阶段对双孢蘑菇中麦角甾醇合成的调控作用，明确MeJA调控双孢蘑菇中麦角甾醇合成的靶向作用，揭示MeJA诱导双孢蘑菇中麦角甾醇积累的分子机制，为生产高麦角甾醇含量的优质双孢蘑菇提供理论基础和科技支撑。

Agaricus bisporus is the most widely cultivated, most productive, most commonly consumed edible mushroom. Ergosterol is the representative functional constituent and provitamin D but low level in Agaricus bisporus, and limited information is available on improving ergosterol content in Agaricus bisporus. In our previous study, we have found that methyl jasmonate （MeJA) treatment can significantly increased the content of ergosterol in Agaricus bisporus, but the molecular mechanism underlying this remains unclear. This project is proposed based on this scientific problems and arranged accodding to the research strategy of "MeJA treatment → inducing gene expression → regulating protein expression → related enzymes activities → promoting the accumulation of ergosterol". The effect of MeJA treatment on the dynamic changes of ergosterol level in Agaricus bisporus will be researched, and the the transcriptional level, protein expression levels and synthetase activity will be investigated using transcriptomics, proteomics, and enzymology. The aims of this project are to illuminate the targeting sites clearly MeJA regulating ergosterol synthesis and revealing the molecular mechanisms of MeJA inducing Agaricus ergosterol accumulation in Agaricus bisporus. These findings will contribute to a theoretical basis and technical strategies for the procution of Agaricus bisporus of high ergosterol quality.