溃疡性结肠炎（UC）的发生发展与肠黏膜屏障破坏密切相关。TNF-ɑ/Elk-1/MLCK通路异常激活介导的细胞紧密连接破坏是肠黏膜屏障受损的重要调节机制之一。大肠湿热证为UC的主要证型，清热化湿法为重要治则。清化肠饮是名老中医杨春波经验方，主治大肠湿热型UC，治疗效果显著。课题组前一个国家自然科学基金项目研究表明清化肠饮可明显改善UC小鼠结肠黏膜的组织学损伤并显著降低LPS介导的肠上皮细胞TNF-ɑ的分泌，提示通过调控TNF-ɑ/Elk-1/MLCK通路、继而促进肠黏膜屏障修复可能是清化肠饮治疗UC的重要机制。在此基础上本课题拟通过体内外实验，采用检测跨膜电阻、糖分子探针等方法研究清化肠饮对UC结肠黏膜通透性的影响，并采用Q-PCR、Western-blot等方法研究清化肠饮对该通路活化和细胞紧密连接蛋白表达的影响。研究将助于进一步阐明清化肠饮治疗UC的内在机制，为其临床应用提供实验依据。

The damage of intestinal mucosal barrier is strongly associated with the development of ulcerative colitis (UC). The abnormal activation of TNF-ɑ/Elk-1/MLCK signal pathway has been implicated as a key mediator for the disturbance in the intestinal the disturbance in the intestinal TJ barrier, which contributed to the impaired intestinal mucosal barrier. Qing Hua Chang Yin (QHCY) is a traditional Chinese formula, which has been demonstrated to be clinically effective in the treatment of UC. However, the precise mechanisms behind its anti‑inflammatory effects remain largely unknown. Our previous studies showed that QHCY can ameliorate histologcal damage of intertstinal mucosa in DSS-induced UC mouse model and reduce the secretion of TNF-ɑ in LPS-stimulated differciated Caco-2 cells. Our findings suggest that QHCY treat UC could through reverse the damage of Intestinal mucosal barrier, which involving in TNF-ɑ/Elk-1/MLCK signal pathway. In the present project we will study the in vivo and in vitro effects of QHCY on the intestinal permeability by measurement of transepithelial electric resistance (TEER) and detection of sugar molecular probes. the Effect of QHCY on the activation of TNF-ɑ/Elk-1/MLCK pathway and the expression of cell tight junction protein will be explored by immunohistochemical staining, Western blot and Real-time quantitative RT-PCR. Our findings in this study will provide new insight into the molecular mechanism of the anti-inflammatory activity of QHCY in UC. It will provide further experimental evidence for clinically treatment of UC.