缺血性心脏病与体内细胞凋亡过程密切相关，基于前期标本配穴防治慢性心肌缺血研究成果，拟以慢性心肌缺血模型大鼠为研究对象，选用标本配穴，以单穴针刺为对照，从心脏功能彩超、心肌酶谱及心肌凋亡超微结构等检测指标，观测标本配穴对心肌缺血的协同改善效应。同时结合基因芯片、Western blot、RT-PCR等技术，观察标本配穴对心肌缺血大鼠细胞凋亡相关基因和蛋白表达的影响，同时采用抑制剂LY294002阻断PI3K/Akt信号通路作为对照，观察标本配穴对相关信号通路激活途径的影响，以阐明标本配穴在心肌缺血损伤中的协同改善作用及对凋亡相关基因的调控机制，进而从基因水平验证标本配穴理论的科学性和实用性，为临床腧穴配伍提供科学依据。

Ischemic Heart Disease is closely related to the process of apoptosis in vivo,with points based on the early stage of research resultson about Acupuncture of Combined Biao and Ben Acupoints on chronic myocardial ischemia,inte- nded to chronic myocardial ischemia model in rats for the study,selected the Acupuncture of Combined Biao and Ben Acupoints,with a selection of sample points to a single acupuncture as control,from the detection indicators of the ultrasound cardiac function, myocardial enzymes and ultrastructure of myocardial apoptosis,observing the Acupuncture of Combined Biao and Ben Acupoints of synergy to improve the effect of myocardial ischemia.Simultaneously Combined with gene chip,Western blot,RT-PCR techniques to observe the affect of apoptosis related genes protein expression of Acupuncture of Combined Biao and Ben Acupoints on myocardial ischemia,while using inhibitor LY294002 to block the PI3K/Akt signaling pathway as a control sample,observed the effect of Acupuncture of Combined Biao and Ben Acupoints on related signaling pathways,to clarify the synergistic improvement effect of the Acupuncture of Combined Biao and Ben Acupoints on ischemic myocardium damaging and the regulatory mechanisms of apoptosis-related genes,then to prove that scientific and practical of the Acupuncture of Combined Biao and Ben Acupoints theory from the genetic level,while to provide a scientific basis for clinical acupoints compatibility.