ATP作为骨骼肌收缩时将化学能转变为机械能的唯一直接能源，影响着机体的运动机能水平。运动力竭后骨骼肌线粒体中与抗增殖蛋白1存在相互作用的蛋白质主要为F0F1-ATP酶的亚单位，而该酶是ATP合成的关键酶。基于对氧化应激致细胞损伤的信号传导通路和线粒体机制获得的深刻认识，结合对目前有关研究资料的系统分析，拟检测不同能量代谢下抗增殖蛋白1的表达；改变骨骼肌内抗增殖蛋白1含量，观察ATP含量、氧化应激及运动能力的变化，研究其在能量代谢中的作用；调控细胞内抗增殖蛋白1含量，观察其对F0F1-ATP酶含量及活性的影响；研究抗增殖蛋白1与F0F1-ATP酶不同亚单位之间的相互作用，以探讨其在调控线粒体F0F1-ATP酶中的作用机制，从而求证：抗增殖蛋白1通过直接或间接途径调节线粒体F0F1-ATP酶亚单位，从而调控F0F1-ATP酶的功能，影响细胞内ATP含量，在运动能量代谢中发挥重要作用。

As the only direct energy resource transforming chemical energy into mechanical energy, ATP affects the movement function of the body. The main protein interacting with prohibitin 1 is the subunit of F0F1-ATP synthase in skeletal muscle mitochondria after exercise exhaustion. F0F1-ATP synthase is the key enzyme of ATP synthesis. Based on the knowledge about the signaling pathways of oxidative stress-induced cell damage and its mitochondrial mechanism and combining with systemetic analysis on the current research of prohibitin 1, the followings will be studied in the present study. The prohibitin 1 expression will be detected under different energy metabolisms. Through changing the prohibitin 1 content in skeletal muscle cells, changes of the ATP content, oxidative stress and exercise capability will be observed and the role of prohibitin 1 will be studied in the energy metabolism. Through regulating the prohibitin 1 content in cells, the influence of the prohibitin 1 content on the enzyme content and activity of F0F1 - ATP synthase will be observed. The interaction of prohibitin 1 with different subunits of F0F1 - ATP synthase will be studied to explore its mechanism of regulating mitochondrial F0F1 - ATP synthase. The followings will be proved in the present study: prohibitin 1 plays an important role in energy metabolism during exercise in terms of modulating the function of F0F1 - ATP synthase and influencing the ATP content of cells through modulating the subunits of this enzyme directly and indirectly.