MYB和bHLH类转录因子是丹参酚酸类成分生物合成的重要调控因子，本团队研究发现：SmMYB39是丹酚酸合成的负调控转录因子，含有bHLH结合结构域，SmbHLH7是丹酚酸合成的正调控转录因子，含有MYB结合结构域。推测SmMYB39可能与SmbHLH7互作，协同调控了丹酚酸的生物合成，但其分子机制尚不清楚。本项目将在前期基础上，主要开展：①通过酵母双杂交、免疫共沉淀和双分子荧光互补技术，体内外鉴定SmMYB39与SmbHLH7的相互作用，确定二者互作区域；②利用染色质免疫共沉淀和凝胶阻滞实验鉴定SmMYB39和SmbHLH7协同调控的靶基因，分析SmMYB39和SmbHLH7复合体的转录激活功能；③采用基因过表达和RNA干扰技术，研究SmMYB39和SmbHLH7基因功能，阐明SmMYB39与SmbHLH7协同调控丹酚酸生物合成的分子机制，为揭示丹参药材品质的形成机制和分子育种奠定基础。

MYB and bHLH transcription factors are important regulators of phenolic acids biosynthesis in Salvia miltiorrhiza. We found that SmMYB39 was a negative regulator of phenolic acids biosynthesis and contained a bHLH-binding domain.SmbHLH7 a positive regulator of phenolic acids biosynthesis and contained a MYB interaction region. We speculated that SmMYB39 probably interacted with SmbHLH7 and regulated the biosynthesis of phenolic acids. However, the molecular mechanism is still unclear. On the basis of our previous work, we will find that ①Identify the interaction and the binding domain of SmMYB39and SmbHLH7 by Yeast two hybrid, Co-IP and BiFC technology; ②Analyze transcriptional activation and identify target genes of SmMYB39-SmbHLH7 by ChIP and EMSA;③Investigate function of SmMYB39 and SmbHLH7 to reveal regulation mechanism of the complex on phenolic acids biosynthesis by gene overexpression and RNAi. Finally, we will demonstrated molecular mechansim of SmMYB39 and SmbHLH tanscription factor on phenilic ancids biosynthesis in Salvia miltiorrhiza, and lay the foundation for uncovering the quality formation mechanism and molecular breeding of Salvia miltiorrhiza.