如何将生物大分子高效、准确且保持活性地运输至活细胞内仍是细胞生物学研究中的关键性技术难题之一。纳米科技的发展为解决该问题提供了多种有效研究手段。在动物细胞生物学基础研究领域，纳米材料已成为实验室常用的，协助分子跨膜转运的有力工具。然而，致密的植物细胞壁阻碍多种无机或有机载体进入细胞，这使得在植物细胞与分子生物学研究领域可供选择的技术手段相对贫乏。我们在前期制备了厚度0.5nm，直径20-50nm的Mg/Al阳离子类水滑石二维片层材料（LDH），并发现LDH具有高效吸附DNA等生物活性分子，且能轻易透过植物细胞壁，携带核酸和荧光染料等进入完整植物细胞中的独特能力。本研究将进一步揭示LDH跨膜转运的分子机制，建立LDH在细胞中转运的分子模型。在此基础上，改造LDH结构，优化实验方法，以期为植物分子和细胞生物学研究提供一种新的分子载体，也将为农林业中重要的非模式植物提供新的细胞转化方法。

One of the most challenging technical problems in the research of cell biology is to transfer the biomolecules into the intact cells with high efficiency and accuracy. The advanced nanotechnology provided various resolutions to conquer this difficulty. In the field of animal cell biological researches, the nanomaterials have been introduced into the routine laboratory works as efficient molecular transmembrane transporters. However, the compact plant cell walls prevented these transporters for reaching the cell membrane. Therefore, only limited approaches were provided in the research of plant molecular and cellular biology. In our previous works, we synthesized layered double hydroxide nanosheets (LDH) with 0.5 nm thickness and 20-50 nm diameters. We found that these LDH based on Mg/Al cations can absorb the DNA and other negative charged molecules with high efficiency. Furthermore, it can easily penetrate the cell wall and transfer these molecules into intact plant cells. In this project, we will study the molecule mechanism of LDH transmembrane transport and establish a computational model for the LDH transmembrane transport. Based on this model, we will improve the formation of the LDH nanosheets and provide an optimal experimental approach for the research of plant molecule and cell biology. Moreover, this method may be widely used the transformation of non-model plants in agriculture and forestry applications.