大面积创伤修复是众多临床皮肤疾病中最常见且最具挑战性的问题之一。虽然皮肤组织工程为解决创面修复提供了一种比较优越的方法，但目前在利用组织工程材料修复大面积创伤的过程中，仍然存在创面愈合时间过长，愈合效率较低等问题，其中一个主要原因就在于人工修复材料往往生物活性较差，不能有效地促进皮肤组织细胞的增殖、分化以及促创面愈合相关因子的表达。为了有效解决这个问题，本项目拟结合静电纺丝技术和激光脉冲沉积法，通过在静电纺丝支架表面引入微图案结构以及纳米生物活性玻璃涂层，制备具有可控的多级微纳米结构的有机/无机复合电纺纤维材料，并进一步研究电纺纤维材料的微纳米级图案结构以及生物玻璃活性成分的协同作用对于皮肤组织细胞的增殖、分化以及促创面愈合相关生长因子表达的影响及其机理，最后采用动物模型验证该材料对于体内创面修复的速率以及质量。本项目将为解决组织工程领域中大面积创伤难以愈合等关键问题提供新思路和新方法。

The regeneration of large skin tissue defects is one of the most challenging issues clinically. Skin tissue engineering approach has come into focus as an alternative source for large skin tissue regeneration. However, up to now, how to shorten the wound healing time and improve the quality of healing is still the most challenging problem, in which one of the major reasons is the poor biological activity of the artificial skin tissue scaffolds. For solving this problem effectively, the aim of this project is to fabricate a new kind of electrospun nanofibrous scaffolds with improved bioactivity. By using the combination of electrospinning and pulsed laser deposition (PLD) methods, the patterned electrospun scaffold with surface coated with nano-sized bioglass were prepared, and the influence of the controlled hierarchically micropatterns in association with the nano-sized bioglass on the cell adhesion, proliferation and differentiation was studied. Finally, the stimulative effect of this new kind of electrospun sacffolds on wound healing in vivo was verified. The significance of this project is that the study will provide novel methods to potentially solve the issues of insufficient wound healing of artificial scaffolds in the skin tissue engineering.