拟南芥蛋白磷酸酶6 (PP6) 是多种发育途径中的分子调控中枢，但其参与植物暗形态建成的功能及分子作用机理至今仍鲜为人知。PIF类转录因子（PIF1、PIF3、PIF4、PIF5）是促进植物暗形态建成的关键调控因子，其功能受磷酸化修饰调控，但催化PIF去磷酸化的磷酸酶和维持PIF在暗环境中稳定性的分子机制仍不明了。本项目组前期研究发现PP6在暗中表达积累并与PIF3互作进而催化PIF3去磷酸化。在此基础上本项目拟研究：（1）PP6调控植物暗形态建成的功能；（2）PP6对PIF1、PIF4、PIF5去磷酸化和稳定性的调控；（3）PP6与PIF在植物暗形态建成中的遗传互作。暗形态建成是种子出苗必经的发育阶段。本项目研究内容的完成，将揭示关键磷酸酶调控植物暗形态建成的功能并完善暗形态建成的分子调控网络，为农作物通过遗传改良来提高种子出苗率的生产实践提供理论基础。

Arabidopsis protein phosphatase 6 (PP6) is a molecular hub in regulating many developmental processes, but to date the function of PP6 in regulating plant skotomorphogenesis and the molecular mechanism remain unknown. PIF transcription factors are important positive regulators promoting seedling skotomorphogenesis. Phosphorylation modification plays a key role in regulating PIF stability and function, but the phosphatase responsible for dephosphorylating PIF protein and the molecular mechanism of keeping PIF stable in darkness remain to be elucidated. In this project, we propose to study (1) the function of PP6 in regulating plant skotomorphogenesis, (2) the function of PP6 in regulation of PIF dephosphorylation and stability and (3) the genetic interactions between PP6 and PIF in regulating plant skotomorphogenesis. Yield increase is premised on the basis of emergence of seedling. Skotomorphogenesis is a fundumental developemntal stage for emergence of seedling. The accomplishment of this project will uncover the important function of a key protein phosphatase in regulating plant skotomorphogenesis, improve the regulatory network of plant skotomorphogenesis on one hand, and provide theoretic guidance for agricultural genetic breeding programs seeking to promote the rate of emergence on the other hand.