逆境诱导蛋白对保护植物失水细胞结构以及逆境敏感生物分子起到重要作用，对参与逆境胁迫的信号转导和基因表达，起到调控作用。大量文献和我们近期研究表明，小麦遭受逆境胁迫会表达大量脱水蛋白，但其上游的调控机制和脱水蛋白功能尚不完全清楚。我们已经克隆到水分胁迫下郑引1号小麦脱水蛋白1-2（WZY1-2）、WZY2基因的启动子，获得RNAi-wzy2转基因小麦，从其转录组的差异表达谱中筛选出一些mRNA，并对其作用进行了初探。本课题在此基础上，深入研究小麦不同亚型脱水蛋白WZY1-2、WZY2的逆境应答机制。揭示小麦WZY1-2、WZY2基因启动子的相关逆境调控因子；解析WZY1-2和WZY2蛋白构象与干旱的关系；筛选鉴定WZY1-2和WZY2的互作蛋白；探明WZY1-2、WZY2表达对小麦不同生育期干旱胁迫的贡献。该项目的完成对于阐明小麦逆境诱导相关蛋白的功能和机理具有重要的理论意义。

The stress-induced proteins play an important role in protecting structure of the cells losing water and sensitive biological molecules, and participate in signal transduction, gene expression and regulation in stress. A large number of literature and our recent studies show that wheat expresses dehydrins in stress. At present, it is not clear how upstream regulatory mechanism and function of dehydrins work. The promoters of WZY1-2 and WZY2 were cloned from wheat zhengyin-1 in water stress. RNAi-WZY2 transgenic wheat is obtained, and mRNA molecules were screened from the different trans criptome expression profile in transgenic wheats. Based on research above, the further study will focus on stress response mechanism of different hypotype of dehydrins WZY1-2 and WZY2in wheat, reveal the function of regulation factors and relationship between WZY1-2, WZY2 proteins’ conformation and drought, screen molecular chaperones interacted with dehydrins WZY1-2 and WZY2, and verify WZY1-2 and WZY2 expression's contribution at different development stages of the wheat in drought stress. The completion of the project will have theoretical significance in the function and mechanism of wheat protein induced in stress.