"娜龙"是黎药中最为常用的草药类群之一，具有祛风散寒、舒筋强骨、活血化络、消肿止痛的功效，被用于跌打损伤、疲血肿痛、骨折、风湿性关节炎等病症的治疗，药用历史悠久，疗效独特。调研发现，"娜龙"类黎药来源复杂，同名异物和同物异名现象突出，甚至出现中毒致死个案。近年来，DNA条形码技术作为传统中药鉴定学的有益补充，被广泛应用于易混淆品种及民族药的基源鉴定，本研究拟以海南五指山、白沙、保亭等黎族地区"娜龙"类中草药调查为基础，综合应用中药鉴定学、DNA条形码及化学指纹图谱等分析手段与方法，对"娜龙"类中草药进行品种整理及用药规律研究，为保证"娜龙"类本草的用药安全提供理论支持，为促进黎药资源的保护、挖掘和可持续利用奠定基础。

"Nalong" is one of the most representative herbs in commonly used Li ethnopharmacology medicines, different forms and modes of preparation of "Nalong" are used for the treatment of unopened wound, colds, back pains, rheumatism, chronic pus, diarrhea and even to eliminate intestinal worms. In the prophase of investigating the ethnopharmacology of Li in Hainan, we discovered the herb of "Nalong" is more than one resource, some varieties used by Li has potential toxicity. In recent years, DNA boarding, as a useful tool of variety identification, was widely used in identification of confused variety, Chinese traditional herbs, traditional herbs used by folk knowledge systems etc. In this research, the resources of "Nalong" would be widely investigated, and also the methods of plant identification, DNA barcoding and chemical fingerprinting would be used in identified the security resources of "Nalong", and with the aim to protect the folk medicine knowledge of Li in Hainan.

项目背景：“娜龙”是黎药中最为常用的草药类群之一，具有祛风散寒、舒筋强骨、活血化络、消肿止痛的功效，被用于跌打损伤、疲血肿痛、骨折、风湿性关节炎等病症的治疗，药用历史悠久，疗效独特。调研发现，“娜龙”类黎药来源复杂，同名异物和同物异名现象突出，甚至还有因服用某些品种中毒致人死亡的报道。近年，DNA条形码技术作为传统中药鉴定学的有益补充，被广泛应用于易混淆品种及民族药的基源鉴定，本研究拟以海南五指山、白沙、保亭等黎族地区“娜龙”类中草药调查为基础，综合应用中药鉴定学、DNA条形码及化学指纹图谱等分析手段与方法，对“娜龙”类中草药进行品种整理及用药规律研究，为保证“娜龙”类中草药的用药安全和黎药资源及文化的保护奠定基础。研究内容：对“娜龙”类本草进行基原调查，并对“娜龙”类本草的黎族用药规律、化学鉴定等进行研究。重要成果：对海南省白沙县、琼中县和五指山市的娜龙类本草进行了基源调查，并利用nrDNA 的ITS序列和cpDNA 的trnL-F序列开展了分子系统学和DNA 条形码研究，此外亦利用GC-MS和LC-MS对艾纳香中挥发性成分和黄酮类成分进行了鉴定，还利用小鼠实验模型，对艾纳香油的安全性和治疗烧烫伤的机制机理进行了研究。科学意义：为“娜龙”类本草的安全使用应用和拓展应用奠定了基础。