糖尿病足致残致死率高，属中医"脱疽"范畴。西医扩血管、抗感染等对症治疗，中医多用活血通脉、清热解毒治之，但血管再生较难。内皮祖细胞（EPCs）可修复血管，减轻炎症。著名中医外科专家赵永昌积60载经验，提出糖足以血脉瘀阻夹热毒型为多，创"补消法-赵氏脱疽方"。该方能减轻炎症，改善微循环。在中医整体观和系统生物学指导下，以临床为中心，血管-炎症为主轴，探讨补消法通过非线性p53-AMPK-miRNA网络，miRNA调控EPCs促进血管再生和减轻炎症的机制。流式细胞术鉴定检测EPCs，分别用RT-PCR，MicroRNA、LncRNA、蛋白芯片测试miRNA、LncRNA、p53及AMPK并用数据构建网络，用高内涵分析EPCs的增殖、分化、迁移、自噬和凋亡的动力学变化，生物信息学和组学解析结果。揭示补消法的科学内涵，阐明与血管-炎症相关疾病的共同发病机制，为科学地制定相应的治疗策略奠定理论基础。

Diabetic foot, which refers to "Tuo Ju" in Traditional Chinese Medicine (TCM), a major cause of the diabetic in maiming and death. Usually, its threraputic strategy to vasodilate and anti-infection in west medicine for promoting blood circulation, however, using removing blood stasis, clearing heat and removing toxicity in TCM. Consequently, it is difficult to make vascular regeneration. However，EPCs can repair the blood vessels and antiphlogosis. With sixty years clinical experience, our famous TCM surgeon Yongchang Zhao proposed that the diabetic foot is main caused by blood stasis and heat-toxicity. By determination of treatment based on the syndrome differentiation, he created "bu-xiao tenet" and "Zhao's prescription", which has a good effect in controling inflammation and improving microcirculation. Under the guidance of integrating the holistic theory of TCM and the systems biology, we will focuse on the clinical and take blood vessels-inflammation as the main axis. And then we explore the mechanism of the endothelial progenitor cells (EPCs) in promoting angiogenesis and diminishing inflammation, through the p53-AMPK-miRNA network. We detect miRNAs, LncRNAs, proteins in the hub of AMPK and genes in the hub of p53 using Micro-RNA, Lnc-RNA and protein, gene microarray chips respectively to be constructed a network. Moreover, EPCs are detected by flow cytometry. Data of RT-PCR and microarray chips are analysed by the correspoding softwares in bio-informatics underlying the theory of the Systems biology. Profles and images of Proliferation, differentiation, apoptosis and autophagy of EPCs are screened on (ImageXpress) High content, genome epigenomics proteonomics are analysed by the corresponding softwares in bio-informatics underlying the theory of the systemic biology. It is a proposal to reveal the scientific connotation of this tenet in TCM, to shed light on blood vessels-inflammation serve the common pathogenes related diseases e.g. diabetes, cancer, cardiovascular dieases and even virous disease which imply the theoretical signifecation, provating the seris of theraputic strategies underlie the theoretic base.

糖尿病足具有致死率、致残率高的特点，属中医“脱疽”范畴。西医以扩血管、抗感染等对症为主，中医多用活血通脉、清热解毒法治之，但血管再生较难。内皮祖细胞（EPCs）可修复血管，减轻炎症。著名中医外科专家赵永昌积60载经验，提出糖足以血脉瘀阻夹热毒型为多，创“补消法-赵氏脱疽方”。该方能减轻炎症，改善微循环。在中医整体观和系统生物学指导下，以临床为中心，血管—炎症为主轴，探讨减轻炎症的机制。课题组成员肖智慧硕士毕业论文《“赵氏方”对血脉瘀阻型DF患者的临床干预及外周血EPC的影响》通过外周血分离并培养EPCs流式细胞仪，检测外周血内皮祖细胞相关标志物的表达情况，对临床试验组患者治疗前后的临床情况进行采集、统计、分析。研究发现血脉瘀阻型DF患者外周血红细胞的数量和血红蛋白的含量较健康人低，验证了“赵氏脱疽方”可增加该类患者外周血红细胞的数量和血红蛋白的作用。其作用机制可能是通过刺激血液系统，促进病变局部侧枝循环的形成，增加局部血液代谢，减轻局部损伤，促进组织修复，从而延缓糖尿病足的发展进程。肖智慧在中国科技核心期刊《中医药信息》发表了《糖尿病足的中医研究进展》，概括的说明了近年来中医对糖尿病足的认识和最新的研究方向及成果，阐述了“赵氏脱疽方”（包括内服用的“赵氏脱疽汤”和外用的“赵氏脱疽膏”）的理论基础。戴娜论文《实验性糖尿病足大鼠模型的研究进展》发表在《长春中医药大学学报》，为下一步动物实验打下基础。课题组一直致力于提高外周血中EPCs提取量，以求符合最终用RT-PCR，MicroRNA、LncRNA、蛋白芯片测试miRNA的要求，经过多次改进实验步骤，达到每25ml外周血提取1350个内皮组细胞，符合下一步测序要求。同时，课题组进行了糖尿病相关T淋巴细胞分群实验分析比较，得出具有统计学意义的结论。