运用有效的康复治疗手段，减轻或消除脑缺血后神经再生的抑制作用，促进神经功能恢复，是脑缺血损伤与修复机制研究新的热点。本项目基于中医经络学说和现代康复理论，在前期促进神经可塑性研究的课题基础上，以电针联合丰富康复训练的疗法与神经再生抑制因子的关联性为切入点，以勿动蛋白及其受体复合体为靶点，通过复制大鼠脑缺血模型，在早期不同时间点分别施以电针疗法、丰富康复训练以及电针联合丰富康复训练的手段，评估上述疗法对大鼠运动、感觉和学习记忆等神经缺损功能的影响，借助免疫组化、分子生物学、病理学、显微图像分析等技术，比较上述三种方法对模型大鼠神经再生抑制因子勿动蛋白、勿动蛋白受体复合体及相关信号通路的影响，以探讨其调控神经再生的可能靶点和机制，为传统康复疗法与现代康复技术的临床结合运用提供新的实验依据和理论基础。

Study of effective rehabilitative treatments on nerve regeneration inhibitory effect to promoting neural function restoration after cerebral ischemia has provided being a hot spot. The project is intended to do an exploring research on nerve regeneration inhibitory effect by means of electroacupuncture therapy combined rich rehabilitation training on treating ischemic stroke. Based on meridians theory of TCM and modern rehabilitation theory, focusing on the Nogo protein and its receptor complex,rat models of local cerebral ischemia will be established, electroacupuncture therapy ,rich rehabilitation training and electroacupuncture therapy combined rich rehabilitation training will be administrated respectively at different time points of earlier period after cerebral ischemia. The different effects on recovery of neural function (motion, sensation, learning and memory)will be estimated. The influences on the nerve regeneration inhibitory factors and the related signalling pathway such as Nogo protein and its receptor complex in rats will be observed via techniques of immunohistochemistry staining , molecular biology ,pathology and micro image analysis . Through these means, the regulatory mechanism of neuroregeneration targeting Nogo protein and its receptor complex will be preliminary approached,experimental evidences and scientific foundation for further application of the traditional Chinese therapy combined the modern rehabilitation technology in the future will be provided.

运用有效的康复治疗手段，减轻或消除脑缺血后神经再生的抑制作用，促进神经功能恢复，是脑缺血损伤与修复机制研究新的热点。本项目基于中医经络学说和现代康复理论，在前期促进神经可塑性研究的课题基础上，以电针联合丰富康复训练的疗法与神经再生抑制因子的关联性为切入点，以勿动蛋白及其受体复合体为靶点，通过复制大鼠脑缺血模型，在早期不同时间点,分别施以电针疗法、丰富康复训练以及电针联合丰富康复训练的手段，观察其对大鼠神经功能的改善作用、对神经再生抑制分子和相关信号转导通路的影响。结果发现：①电针联合丰富康复训练能一定程度上促进局灶性脑缺血后大鼠神经功能恢复，优于单纯电针治疗与丰富康复训练；②电针联合丰富康复训练能够缩小局灶性脑缺血大鼠脑梗死体积，优于单纯电针治疗与丰富康复训练；③电针联合丰富康复训练能提高局灶性脑缺血大鼠平衡、行走及抓握能力，增加患肢肌力优于单纯电针治疗，在提高平衡能力方面优于丰富康复训练；④电针联合丰富康复训练能够改善大鼠缺血区皮层和海马神经元、突触超微结构和脑组织其他病理改变，优于单纯电针治疗与丰富康复训练；⑤电针联合丰富康复训练能够下调大鼠缺血区皮层和海马NgR、LINGO-1、p75NTR、TROY及其Nogo信号通路下游蛋白RhoA、ROCK的表达，降低勿动蛋白受体复合体的形成，从而促进神经功能恢复，这可能是电针联合丰富康复训练促进脑缺血后神经功能重塑的相关机制之一。通过以上实验结果以及对调控神经再生的又一可能靶点和机制的探讨，为传统康复疗法与现代康复技术的临床结合运用提供了新的实验依据和理论基础。