中文摘要
针对窦房结起步兴奋节律这一重要科学问题，选取起步心肌细胞兴奋节律的形成和变化机理这一关键内容，从生理学和非线性动力学交叉的角度，研究心脏起步细胞自律性的形式多样性和变化规律性。项目提出心脏起步细胞复杂自律性的学术观点，从窦房结起步单细胞、起步细胞团等层次，依据非线性动力学概念科学地归纳与定义实验观察到的多种起步节律模式；通过实验发现在重要生理参数驱动下起步节律的转迁规律；应用并搭建贴近实际的动力学模型，结合实验进行仿真和理论分析，揭示多种自动兴奋节律形成和节律间转迁的动力学和生理学机理；研究缝隙连接电流对自律性的影响。积极探索起步细胞的复杂自律性在窦房结的生理性和部分病理性的起步活动中的基础意义。研究结果将加深对窦房结兴奋机制的理解，对起步细胞自律性异常导致的病理性心律的形成机制进行新的探讨，为进一步深刻、全面认识窦房结自动兴奋的生理学与生物物理学原理奠定基础。
英文摘要
The present project studies the mechanisms underlying the formation of diverse rhythms of cardiac cell excitation, as well as the transitions between different rhythms. It aims at the understanding for autorhythmicity of sinus atrial node. Physiological experimentation and nonlinear analysis are integrated in the studies of the rhythmic diversity and the regularity of rhythm transition. A standpoint of complex rhythmicity is propounded based on previous studies and is to be further tested for at single pacemaker cell and cell cluster levels. The observed rhythms is going to be identified using dynamic definitions such as periodic, chaotic, and stochastic, and the rhythm transtions driven by physiological parameters will be judged from the perspective of bifurcation scenarios. We also plan to modify or construct dynamic mathematical models of the problem, and simulate and analyze the dynamics underlying the experimental phenomena. The coupling between pacemakers will be evaluated in the study of the adjustment of rhythmicity via gapjunction current. The significance of complex autorhythmicity for a deeper understanding of SAN rhythmgenesis and for pathological rhymicity, such as sinus arrest, will also be emphasized, in a systematic evaluation from the perspective of quantitive biophysics.
结题摘要
针对窦房结起步兴奋节律这一重要科学问题，选取起步心肌细胞兴奋节律的形成和变化机理这一关键内容，从生理学和非线性动力学交叉的角度，研究心脏起步细胞自律性的形式多样性和变化规律性。项目提出心脏起步细胞复杂自律性的学术观点，从窦房结起步单细胞、起步细胞团等层次，依据非线性动力学概念科学地归纳与定义实验观察到的多种起步节律模式；通过实验发现在重要生理参数驱动下起步节律的转迁规律；应用并搭建贴近实际的动力学模型，结合实验进行仿真和理论分析，揭示多种自动兴奋节律形成和节律间转迁的动力学和生理学机理；研究缝隙连接电流对自律性的影响。积极探索起步细胞的复杂自律性在窦房结的生理性和部分病理性的起步活动中的基础意义。研究结果将加深对窦房结兴奋机制的理解，对起步细胞自律性异常导致的病理性心律的形成机制进行新的探讨，为进一步深刻、全面认识窦房结自动兴奋的生理学与生物物理学原理奠定基础。