本项目在发现了普通谷物淀粉中存在纳米层次的基本组成单元和这些基本组成单元的拓扑结构的丰富多样性，并建立了谷物淀粉纳米结构单元的质谱解析方法的基础上，利用分子操纵和酶解技术，针对中国谷物中的优势、特殊品种：高支链玉米、小麦的淀粉颗粒进行修饰，使其解离出基本组成中的纳米结构单元；分类了原子力显微镜所观测的高支链玉米、小麦淀粉纳米结构单元的形态；利用质谱仪、X-射线衍射仪等方法解析淀粉纳米结构单元的分子量范围、多糖组成序列、晶体结构和聚合特征，探索高支链特种淀粉纳米结构单元的自有规律。通过比较特种玉米、小麦淀粉与普通玉米、小麦淀粉纳米组成单元的差异，深化对特种玉米、小麦淀粉流变学品质的认识与评价；通过发现特种玉米、小麦与普通玉米、小麦淀粉纳米结构单元的异同与种质之间的关系，探索使用生物工程技术控制谷物淀粉形态的可行性，以新的发现和成果拓展前期的研究，实现持续性创新。

Based on the fact that nano-level units exist in cereal starch, the topology structures of these units are diverse, and the MS(mass spectrometry) analytical method of cereal starch nanostructure is established, the project modified the special varieties in China cereals, including high branched-chain corn, wheat starch particles by molecular manipulation and enzyme technology. The nano-structural units of the basic components are separated, and the morphology observed by AFM(atomic force microscope) of high branched-chain corn and wheat starch is classified. These more accurate separation and classification make the starch scientific system be strengthened. The molecular weight range and crystal structure of starch nano-structural units are obtained using MS and X-ray diffraction analysis methods, then the concept of polysaccharide "sequence" is proposed, which supply a new foundation for studying the nano-structures and characteristics of cereal starch. By comparing nano-structural units of the special corn, wheat starch and normal corn, wheat starch, we deepen the understanding and evaluation of rheology quality about special corn, wheat starch, which is good for exploring its own law of special starch nano-structural units. By studying the differences between the special corn, wheat and normal corn, wheat starch nano-structural units, we explore the feasibility that using bio-engineering technology to control morphology of cereal starch, and promote industrial development with a relevant scientific and technological projects. On the basis of new discoveries and achievement, preliminary studies is developed and a sustained innovation is finished, which provide new targets and ideas for a new economic growth point about the development of cereal production and cereal starch processing industry.

本项目在发现了普通谷物淀粉中存在纳米层次的基本组成单元和这些基本组成单元的拓扑结构的丰富多样性，并建立了谷物淀粉纳米结构单元的质谱解析方法的基础上，利用分子操纵和酶解技术，针对中国谷物中的优势、特殊品种：高支链玉米、小麦的淀粉颗粒进行修饰，使其解离出基本组成中的纳米结构单元；分类了原子力显微镜所观测的高支链玉米、小麦淀粉纳米结构单元的形态；利用质谱仪、X-射线衍射仪等方法解析淀粉纳米结构单元的分子量范围、多糖组成序列、晶体结构和聚合特征，探索高支链特种淀粉纳米结构单元的自有规律。通过比较特种玉米、小麦淀粉与普通玉米、小麦淀粉纳米组成单元的差异，深化对特种玉米、小麦淀粉流变学品质的认识与评价；通过发现特种玉米、小麦与普通玉米、小麦淀粉纳米结构单元的异同与种质之间的关系，探索使用生物工程技术控制谷物淀粉形态的可行性，以新的发现和成果拓展前期的研究，实现持续性创新。