本课题旨在设计利用氧化还原电位调控及膜分离技术的新型厌氧生物反应装置应用于厌氧条件下菌株固定二氧化碳合成丁二酸的体系。本课题研究了氧化还原电位调控对于菌株生长以及丁二酸合成的关键酶的影响，确定了有利于丁二酸合成的氧化还原电位的发酵调控策略；针对产丁二酸过程的特点，采用微滤、纳滤构建膜生物反应器，实现了反应-分离过程耦合，减少了有机酸对发酵过程抑制作用，实现了丁二酸高生产强度制备；综合利用反应器的控制与氧化还原电位调节的手段，同时将基于膜分离的原位提取技术引入丁二酸的生物合成过程中，对生物反应体系中的底物与产物浓度、氧化还原电位、压力、温度、pH等重要参数进行考察与调整，补料分批发酵丁二酸产量最高可达83.5g/L，质量收率达到81.2%，生产强度达到3.1g/(Loh)；连续发酵丁二酸的生产强度达到4.0 g/(L h)，质量收率达到83.5 %， 实现了丁二酸高生产强度、高收率的制备。本课题实施过程中共发表论文37篇，其中SCI收录5篇，EI收录 4篇，申请发明专利11项，其中2项授权，共培养硕博士研究生17名。课题组经过三年的努力，圆满完成了研究任务，达到了预期的研究目标。

The aim of the project was to realize the efficient production of succinic acid by CO2 fixation by using novel anaerobic fermentation device which contained redox regulation and membrane separation system. The effects of redox on the cell growth and key enzymes for succinic acid production were investigated, and the favorable strategy for succinic acid production by redox regulation has been established. According to the characteristics of succinic acid fermentation, a microfiltration-nanofiltration membrane reactor was also set up for coupling the fermentation and separation. As a result, the inhibition of the cell growth and succinic acid production from organic acids accumulation was reduced and succinic acid productivity was improved. The concentrations for the substrates and products, redox, pressure, temperature and pH in the fermentation progress could be successful controlled by combining reactor control, redox regulation and membrane separation system in the novel anaerobic fermentation device. At the optimized conditions, succinic acid concentration of 83.5g/L was obtained with the yield of 81.2% and productivity of 3.1g/L h by fed-batch fermentation, while succinic acid productivity of 4.0g/L h with the yield of 83.5% was achieved by continuous fermentation. We have published 37 articles and 11 patents, among which 5 articles have been accepted by SCI, 4 articles have been accepted by EI and 2 of patents have been authorized. There were 17 graduate students who have been trained. Through three-year hard work, we completely achieved the scheduled objects.

本课题旨在设计利用氧化还原电位调控及膜分离技术的新型厌氧生物反应装置应用于厌氧条件下菌株固定二氧化碳合成丁二酸的体系。本课题研究了氧化还原电位调控对于菌株生长以及丁二酸合成的关键酶的影响，确定了有利于丁二酸合成的氧化还原电位的发酵调控策略；针对产丁二酸过程的特点，采用微滤、纳滤构建膜生物反应器，实现了反应-分离过程耦合，减少了有机酸对发酵过程抑制作用，实现了丁二酸高生产强度制备；综合利用反应器的控制与氧化还原电位调节的手段，同时将基于膜分离的原位提取技术引入丁二酸的生物合成过程中，对生物反应体系中的底物与产物浓度、氧化还原电位、压力、温度、pH等重要参数进行考察与调整，补料分批发酵丁二酸产量最高可达83.5g/L，质量收率达到81.2%，生产强度达到3.1g/(Loh)；连续发酵丁二酸的生产强度达到4.0 g/(L h)，质量收率达到83.5 %， 实现了丁二酸高生产强度、高收率的制备。本课题实施过程中共发表论文37篇，其中SCI收录5篇，EI收录 4篇，申请发明专利11项，其中2项授权，共培养硕博士研究生17名。课题组经过三年的努力，圆满完成了研究任务，达到了预期的研究目标。