中文摘要
本项目通过对gonocyte、精原干细胞发育及调节机理研究,取得下列成果:1.建立国际上第一株来自成年C57BL品系小鼠精原干细胞系并揭示其生物学特性。2.发现新颖粘附分子STPB―C通过调节多信号机制促进gonocyte增殖和精原干细胞自我更新。3. 辩认、克隆和特征化另外3个与前体精原干细胞和精原干细胞增殖、分化有关的新基因和新颖基因, 以及辩认、克隆一促进精原干细胞增殖的miRNA。4.揭示雷公藤多甙和肉苁蓉对前体精原干细胞和精原干细胞发育的影响及机理。5. 首次分离、培养和建立了成年及5天小鼠雌性生殖干细胞系并阐明其生物学特性以及与精原干细胞的相似性。6. 建立了多个经生殖干细胞途经转基因和基因沉默的小鼠模型。发表Nature Cell Biology 等SCI论文24篇。本项目不但对进一步了解精子发生、调控机制、开发优良动物品种、不孕症治疗、探索环境因素对生殖发育的影响以及珍稀动物的保存、动物繁殖具有重要意义，而且为避孕药物开发、人口调控、性细胞途径的基因治疗和优生奠定基础。
英文摘要
After the investigation on regulatory mechanisms of gonocytes and spermatogonial stem cells, the results from our project show: 1. for the first time established and characterized a spermatogonial stem cell (SSC) line derived from adult C57/BL mice; 2. The STPB-C, a novel cell adhesion molecule, regulates proliferation of gonocytes and SSC self-renewal via multiple signaling pathways; 3. identified, cloned, as well as characterized three novel genes playing regulatory roles in proliferation and differentiation of SSCs and precursor SSCs, along with identifying and cloning a miRNA which promotes SSC proliferation; 4.revealed the cell physiological effects of multiglycosides of Tripterygium Wilfordii and Cistanche Deserticola in SSC and theirprecursors, as well as the underlying mechanisms; 5. for the first time isolated, maintained and established adult and neonatal mouse female germline stem cell lines, meanwhile delineating their biological characterization as well as similarity with SSCs; 6. established a number of mouse transgenic/gene silencing models by germ-cell-mediated gene modification. A total of 24 SCI-indexed papers have been published, including one on Nature Cell Biology. This project provides significant insights for understanding the regulatory mechanisms in spermatogenesis, breeding, treatment
结题摘要
本项目通过对gonocyte、精原干细胞发育及调节机理研究,取得下列成果:1.建立国际上第一株来自成年C57BL品系小鼠精原干细胞系并揭示其生物学特性。2.发现新颖粘附分子STPB―C通过调节多信号机制促进gonocyte增殖和精原干细胞自我更新。3. 辩认、克隆和特征化另外3个与前体精原干细胞和精原干细胞增殖、分化有关的新基因和新颖基因, 以及辩认、克隆一促进精原干细胞增殖的miRNA。4.揭示雷公藤多甙和肉苁蓉对前体精原干细胞和精原干细胞发育的影响及机理。5. 首次分离、培养和建立了成年及5天小鼠雌性生殖干细胞系并阐明其生物学特性以及与精原干细胞的相似性。6. 建立了多个经生殖干细胞途经转基因和基因沉默的小鼠模型。发表Nature Cell Biology 等SCI论文24篇。本项目不但对进一步了解精子发生、调控机制、开发优良动物品种、不孕症治疗、探索环境因素对生殖发育的影响以及珍稀动物的保存、动物繁殖具有重要意义，而且为避孕药物开发、人口调控、性细胞途径的基因治疗和优生奠定基础。