中文摘要
本研究通过限制性培养从土壤中筛选出两种常温、高效产酶的木质纤维素分解复合菌系XDC-2和WSD-5，用分子生态学的方法确定了其菌种组成，对主要产酶菌株的行为和作用及产酶机理进行了研究。实验表明WSD-5在15天内可分解麦秆75.6%，糖浓度达到单位重量秸秆的10.92mg/g；WSD-5分泌滤纸酶（活性最高为1.3U/mL）、纤维素内切酶（4.35U/mL），外切酶（0.6U/mL），β-葡萄糖苷酶（0.43U/mL）和木聚糖酶（15.16U/mL）。首次发现中温产酶，高温糖化的规律，WSD-5粗酶液对麦秆的糖化率达到单位重量秸秆的190.08mg/g，是WSD-5分解麦秆过程中糖浓度的17倍。SDS-PAGE分析表明WSD-5粗酶液中有两条蛋白条带，其分子质量分别为45.53±0.06 KDa和38.62±0.48 KDa。菌株组合实验表明真菌Coprinus cinereus与细菌B8、C8和D8组合后，其分泌的纤维素内切酶、β-葡萄糖苷酶和木聚糖酶显著高于原复合系所分泌的酶，表明这3株细菌与真菌之间具有协同分解能力，且能够大幅度提高真菌的产酶能力。
英文摘要
Through the restrictive training from soil 2 cellulolytic degrading microbial community XDC-2 and WSD-5 with high efficiency of the enzyme production were selected.With molecular ecology method to determine the composition of the microbial community, and the main enzyme production strains behavior and function and enzyme production mechanism has been studied. Experiments show that after 15 days cultivation at 30℃, the degradation rate of wheat straw by WSD-5 was 75.6%. The highest filter paper activity, CMCase, Avicelase, β-glucosidase and xylanase activities were 1.30, 4.35, 0.60, 0.43 and 15.16 U/mL, respectively. The results of saccharification showed that the saccharification rate was 190.08mg/g, and it was 17 times higher than the concentration of soluble sugar during the degradation of wheat straw by WSD-5. The results of SDS-PAGE showed that two bands was detected in the crude enzyme of WSD-5 and the molecular weight was 45.53±0.06 KDa and 38.62±0.48 KDa, respectively. SDS-PAGE analysis shows that WSD-5 thick liquid enzymes in two bands of protein, the molecular quality were 45.53± 0.06 KDa and 38.62 ±0.48 KDa. Adding coprinus cinereus to the cultual of B8,C8 and D8 showed that the secretion of cellulose enzymes, beta glycosidase enzymes and xylanase significantly higher than the original community.It indicates that the three strains of bacteria and fungi has cooperative between decomposition ability, and can greatly improve the fungi enzyme production ability.
结题摘要
本研究通过限制性培养从土壤中筛选出两种常温、高效产酶的木质纤维素分解复合菌系XDC-2和WSD-5，用分子生态学的方法确定了其菌种组成，对主要产酶菌株的行为和作用及产酶机理进行了研究。实验表明WSD-5在15天内可分解麦秆75.6%，糖浓度达到单位重量秸秆的10.92mg/g；WSD-5分泌滤纸酶（活性最高为1.3U/mL）、纤维素内切酶（4.35U/mL），外切酶（0.6U/mL），β-葡萄糖苷酶（0.43U/mL）和木聚糖酶（15.16U/mL）。首次发现中温产酶，高温糖化的规律，WSD-5粗酶液对麦秆的糖化率达到单位重量秸秆的190.08mg/g，是WSD-5分解麦秆过程中糖浓度的17倍。SDS-PAGE分析表明WSD-5粗酶液中有两条蛋白条带，其分子质量分别为45.53±0.06 KDa和38.62±0.48 KDa。菌株组合实验表明真菌Coprinus cinereus与细菌B8、C8和D8组合后，其分泌的纤维素内切酶、β-葡萄糖苷酶和木聚糖酶显著高于原复合系所分泌的酶，表明这3株细菌与真菌之间具有协同分解能力，且能够大幅度提高真菌的产酶能力。