项目执行期间，利用纳米金还原过程的信号放大效应，成功实现低达0.4 fmol 的DNA检测限和单碱基错配分辨；将一种DNA脱氧核酶改造成传感器信号放大模块并与DNA适配体结合实现低至6 uM 腺苷的选择性检测；以T-T错配作为Hg2+识别单元并与脱氧核酶的催化放大作用结合，实现4.5 nM的 Hg2+检出限；以DNA为模板合成出具有电催化活性的钯修饰电极；在碳管上接枝高杂交活性的DNA序列，实现对碳管聚集状态的可逆调控，得到纳米金在碳管上的一维组装阵列，该结果对发展基于碳管修饰电极的DNA和蛋白传感器有重要意义；构造出可对特定序列 DNA 进行识别、抓取、夹持和释放的功能核酸分子镊；利用DNA将纳米金组装到石墨烯表面，可望用作新型电化学传感界面。上述结果在J. Am. Chem. Soc.、Angew. Chem. Int. Ed.、Adv. Mater.、Small、Chem. Commun.、Analyst、Biosens. Bioelectron.等杂志上发表论文9篇。多次受邀参加国内外学术会议，出版图书1章。邓兆祥教授获中国化学会"青年化学奖"和中国科技大学"优秀青年教师奖"。

During the execution of this project, we achieved the following results: (1) Based on the amplification effect of a gold deposition process, 0.4 fmol DNA was detected with an ability of resolving a single base mutation; (2) A DNAzyme was reengineered into a signal-amplification module and combined with a DNA aptamer to realize a detection of as low as 6 uM adenosine; (3) Taking T-T mismatched basepairs for the recognition of Hg2+ and using a DNAzyme for catalytic signal amplification, as low as 4.5 nM Hg2+ could be detected; (4)An electrocatalytically active Pd nanoparticle modified electrode was prepared with DNA as a template for Pd deposition; (5) Single walled carbon nanotubes were successfully grafted with DNA sequences maintaining high hybridization activities, based on which the aggregation states of carbon nanotubes could be effectively controlled. Gold nanoparticles were successfully assembled onto the DNA modified carbon nanotubes based on DNA hybridizations. This research was of significance for the construction of a carobn nanotube based electrochemical sensor for DNA and protein detections; (6) A pair of nucleic acid tweezers were built, which were capable of recognizing, capturing, holding and releasing a DNA target on demand; (4) Gold nanoparticles were assembled onto DNA decorated basal surface of graphene, with potential use for a new type of electrochemical sensing interface. The above results have resulted in 9 research papers published in journals including J. Am. Chem. Soc., Angew. Chem. Int. Ed., Adv. Mater., Small, Chem. Commun., Analyst and Biosens. Bioelectron. etc. The PI has been invited to attend domestic as well as international conferences several times and has contributed one chapter to a published book. Also, the PI has been recognized by the "Young Chemist Award" of the Chinese Chemical Society, and the "Outstanding Young Faculty" of the University of Science and Technology of China.

项目执行期间，利用纳米金还原过程的信号放大效应，成功实现低达0.4 fmol 的DNA检测限和单碱基错配分辨；将一种DNA脱氧核酶改造成传感器信号放大模块并与DNA适配体结合实现低至6 uM 腺苷的选择性检测；以T-T错配作为Hg2+识别单元并与脱氧核酶的催化放大作用结合，实现4.5 nM的 Hg2+检出限；以DNA为模板合成出具有电催化活性的钯修饰电极；在碳管上接枝高杂交活性的DNA序列，实现对碳管聚集状态的可逆调控，得到纳米金在碳管上的一维组装阵列，该结果对发展基于碳管修饰电极的DNA和蛋白传感器有重要意义；构造出可对特定序列 DNA 进行识别、抓取、夹持和释放的功能核酸分子镊；利用DNA将纳米金组装到石墨烯表面，可望用作新型电化学传感界面。上述结果在J. Am. Chem. Soc.、Angew. Chem. Int. Ed.、Adv. Mater.、Small、Chem. Commun.、Analyst、Biosens. Bioelectron.等杂志上发表论文9篇。多次受邀参加国内外学术会议，出版图书1章。邓兆祥教授获中国化学会"青年化学奖"和中国科技大学"优秀青年教师奖"。