低温冷害会造成水稻大幅度减产，是我国水稻生产、尤其是我国南方稻区所面临的突出问题。针对这一问题，项目围绕江西特有的东乡野生稻能耐受低温而越冬存活的强耐冷性状，选择东乡野生稻为研究对象，研究了东乡野生稻与两种普通栽培稻在整个生育期和人工冷胁迫处理下的耐寒生理，同时比较分析了东乡野生稻苗期叶片中POD同工酶酶谱的特异性以及冷胁迫对该酶谱的影响；克隆获得SFR2基因、Mn-SOD基因、P5CS基因、VC渗透酶基因和NAD7基因。研究发现冷胁迫能诱导POD的表达。构建东乡野生稻Mn-SOD基因和P5CS基因载体，通过农杆菌介导构建转基因水稻，进行转基因水稻研究。这些研究为深入研究东乡野生稻耐冷的分子机理、开发利用东乡野生稻的优质耐冷基因资源、改良水稻耐冷性以提高栽培水稻的耐冷能力奠定基础。

Freeze injury, which causes decrease of rice yield, is critical problem of rice production in our country, especially in southern area. To solve this problem, Physiological Mechanism for cold tolearance of the Dongxiang Wide Rice in whole life period and under the artificial cold stress was studied. The POD isozyme patterns and the differences of POD isozyme pattern under cold stress were analysized. the genes of SFR2, Mn-SOD, P5CS, VC Permease and NAD7 was cloned fron Dongxiang wild rice, and the results showed that POD was induced to express by cold stress , however SFR2 was not; the vectors of Mn-SOD and P5CS was constructed and introduced into the rice of nongda288 for the trangenic rices study. This study will provide theoretic basis for further elucidating the molecular mechanism of chilling tolerance of Dongxiang wild rice and the utilization of its high quality gene resourse for freeze tolerance.

低温冷害会造成水稻大幅度减产，是我国水稻生产、尤其是我国南方稻区所面临的突出问题。针对这一问题，项目围绕江西特有的东乡野生稻能耐受低温而越冬存活的强耐冷性状，选择东乡野生稻为研究对象，研究了东乡野生稻与两种普通栽培稻在整个生育期和人工冷胁迫处理下的耐寒生理，同时比较分析了东乡野生稻苗期叶片中POD同工酶酶谱的特异性以及冷胁迫对该酶谱的影响；克隆获得SFR2基因、Mn-SOD基因、P5CS基因、VC渗透酶基因和NAD7基因。研究发现冷胁迫能诱导POD的表达。构建东乡野生稻Mn-SOD基因和P5CS基因载体，通过农杆菌介导构建转基因水稻，进行转基因水稻研究。这些研究为深入研究东乡野生稻耐冷的分子机理、开发利用东乡野生稻的优质耐冷基因资源、改良水稻耐冷性以提高栽培水稻的耐冷能力奠定基础。