完成了项目计划提出的CO2超临界流体介质中的皮革酶脱毛、酶软化研究，取得了重要研究成果。发现并确认CO2超临界流体介质是一种良好的蛋白质水解反应介质，蛋白质的酶催化水解反应可以在该介质中发生，枯草杆菌蛋白酶和动物胰酶等对多种蛋白质底物显示强烈的水解能力，其水解活力在可比条件下甚至超过常规水环境；研究确认水是酶催化蛋白水解反应的必需添加剂，微量水的存在是酶催化蛋白质水解反应的必备条件，最低水需求量仅为底物质量的10％，过量的水反而降低了水解活力；研究发现，表面活性剂对促进酶和蛋白质底物在介质中的分散性及其相互亲和性有非常重要的影响，表面活性剂可以显著提高酶对蛋白质底物的水解活性、降低反应的临界压力，某些非离子表面活性剂甚至将水解反应的临界压力降到极低的7.5MPa、水解率提高100％以上；在CO2超临界流体介质体系中可以有效实现酶脱毛，且最低酶制剂的需求量只有50u/g，为常规水环境条件的20％，是实施皮革酶脱毛的新的技术途径。研究结果表明：CO2超临界流体介质中的皮革酶脱毛、酶软化具有极大的可能性，为CO2超临界流体介质中的清洁化制革、蛋白质的洁净加工提供了全新的技术和理论指导。

According to the research plan, the enzymatic unhairing and bating under medium of carbon dioxide supercritical fluid(CO2-SCF) has been studied and following important results have been discovered and confirmed..1. CO2-SCF is a good medium for hydrolysis of proteins. The enzymatic hydrolyses of proteins can take place under medium of CO2-SCF, many enzymes including subtilis protease and pancrease have showed high activity of protein hydrolysis. Its activity could be even higher than in water system under some comparable condition. .2. Small volume of water is essential factor of enzymatic hydrolysis under medium of CO2-SCF, the minimum quantity of water could be lower 10% of the substrates and enzymatic activity of protein hydrolysis should been reduced when the quantity of water is excess..3. Surface active agents have important influence to promote the dispersal of proteins and the affinity between enzymes and substrates. They raise the hydrolysis activity of enzymes and reduces the critical pressure of the system for the enzymatic hydrolysis of proteins obviously and non-ion surface active agents have showed the better effects, it reduces the critical pressure of hydrolysis in 7.5MPa and raises the hydrolysis ratio of protein substrates over 100％。 .4. It is possibility to practice the enzymatic unhairing and bating under medium of CO2-SCF and just 50u/g of enzyme are demanded, it is about 20% of the amount of enzymes in water system ..These results suggestion that it has highly possibility for the practice of enzymatic unhairing and bating under CO2-SCF medium and it could be a new way for the clearing technology of leather manufacturing.

完成了项目计划提出的CO2超临界流体介质中的皮革酶脱毛、酶软化研究，取得了重要研究成果。发现并确认CO2超临界流体介质是一种良好的蛋白质水解反应介质，蛋白质的酶催化水解反应可以在该介质中发生，枯草杆菌蛋白酶和动物胰酶等对多种蛋白质底物显示强烈的水解能力，其水解活力在可比条件下甚至超过常规水环境；研究确认水是酶催化蛋白水解反应的必需添加剂，微量水的存在是酶催化蛋白质水解反应的必备条件，最低水需求量仅为底物质量的10％，过量的水反而降低了水解活力；研究发现，表面活性剂对促进酶和蛋白质底物在介质中的分散性及其相互亲和性有非常重要的影响，表面活性剂可以显著提高酶对蛋白质底物的水解活性、降低反应的临界压力，某些非离子表面活性剂甚至将水解反应的临界压力降到极低的7.5MPa、水解率提高100％以上；在CO2超临界流体介质体系中可以有效实现酶脱毛，且最低酶制剂的需求量只有50u/g，为常规水环境条件的20％，是实施皮革酶脱毛的新的技术途径。研究结果表明：CO2超临界流体介质中的皮革酶脱毛、酶软化具有极大的可能性，为CO2超临界流体介质中的清洁化制革、蛋白质的洁净加工提供了全新的技术和理论指导。