本课题以SD大鼠和体外血脑屏障（BBB）模型为研究对象1）以透射电镜和跨内皮电阻抗(TEER)测定为主要手段，检测了电磁脉冲(EMP)辐照后BBB紧密连接（TJ）结构和功能的变化，发现辐照后TEER值明显降低，BBBTJ处可见示踪物质漏出，表明BBB TJ损伤是EMP致BBB开放的重要作用机制；2）采用蛋白杂交、免疫荧光等方法，观察了EMP辐照后TJ 相关蛋白表达和细胞内定位的变化，发现辐照后TJ蛋白表达明显降低，并且细胞内定位也发生了改变，提示TJ 相关蛋白表达异常和细胞内定改变是BBB TJ损伤的主要分子机制；3）通过检测EMP辐照后蛋白激酶C （PKC）及其亚型的蛋白表达情况以及PKC抑制剂H7预处理后BBB通透性和TJ蛋白定位变化，发现辐照后PKC表达明显增加，H7 预处理可明显减轻EMP辐照后BBB通透性的改变，并且部分阻止了TJ蛋白ZO-1 的细胞内定位变化，表明在EMP致BBB开放过程中PKC对TJ相关蛋白细胞内定位发挥重要的调节作用。上述研究结果丰富了EMP的生物学效应理论，为EMP辐射卫生防护提供了思路。

SD rats and in vitro Blood-Brain Barrier (BBB) were used in this study.1) The structure and function of BBB tight junction (TJ)after electromagnetic pulse (EMP) exposure was observed by transmission electron microscope and transendothelial electrical resistance (TEER) assay. It was found that TEER value decreased significantly, BBB tracer was found at TJ place after EMP exposure, which suggested that TJ injury was involved in EMP-induced BBB opening. 2)Results from Western Blot and immunofluorescence showed that the expression and location of TJ associated proteins changed after EMP exposure, which indicated that the alteration of TJ protein expression and location was involved in BBB-induced TJ injury.3)The expression level of PKC increased after EMP exposure, and the inhibitor of PKC (H7) pretreatment mostly inhibited EMP-induced BBB opening, the alteration of TJ protein ZO-1 translocation was also improved, which suggest that PKC plays an important role in EMP-induced BBB opening and ZO-1 translocation. These data provided information for the theory of EMP bio-effect and EMP prevention.

本课题以SD大鼠和体外血脑屏障（BBB）模型为研究对象1）以透射电镜和跨内皮电阻抗(TEER)测定为主要手段，检测了电磁脉冲(EMP)辐照后BBB紧密连接（TJ）结构和功能的变化，发现辐照后TEER值明显降低，BBBTJ处可见示踪物质漏出，表明BBB TJ损伤是EMP致BBB开放的重要作用机制；2）采用蛋白杂交、免疫荧光等方法，观察了EMP辐照后TJ 相关蛋白表达和细胞内定位的变化，发现辐照后TJ蛋白表达明显降低，并且细胞内定位也发生了改变，提示TJ 相关蛋白表达异常和细胞内定改变是BBB TJ损伤的主要分子机制；3）通过检测EMP辐照后蛋白激酶C （PKC）及其亚型的蛋白表达情况以及PKC抑制剂H7预处理后BBB通透性和TJ蛋白定位变化，发现辐照后PKC表达明显增加，H7 预处理可明显减轻EMP辐照后BBB通透性的改变，并且部分阻止了TJ蛋白ZO-1 的细胞内定位变化，表明在EMP致BBB开放过程中PKC对TJ相关蛋白细胞内定位发挥重要的调节作用。上述研究结果丰富了EMP的生物学效应理论，为EMP辐射卫生防护提供了思路。