本研究以水稻齿裂矮缩病毒为模型，研究植物dsRNA病毒的侵染机制。在过去研究的基础上徊街っ鞲弑泶锊《就饪峭黄鸬鞍椎淖蛩荆俏勾纠コ婧螅梢砸种撇《驹诶コ嫣迥诘脑鲋常⒁种撇《镜拇ィ佣醋蛩痉乐尾《镜囊惶跣峦揪叮毖芯棵浇槔コ嫣迥诳赡艽嬖诘牟《臼芴澹圆鞑《厩秩竞筒《?-昆虫--寄主植物相互作用的机啤

Using the Rice Ragged Stunt Oryzavirus, as a research model of plant ds-RNA virus (phytoreovirus) the mechanism of vector transmission of phytoreovirus has been studied. Transgenic rice lines expressing the 39kDa spike protein showed good resistence to infection by RRSV. The insect vector the rice brown plant hopper fed on these plants prior to feeding on RRSV-infected plants were significantly protected against RRSV infection. The results suggested that the 39kDa protein interferes with the interaction between the intact virus and insect cell receptors. The approach would be a more environment-friendly and sustainable method of virus control than by actual eradication of insect vectors. The studies on functions of products of S6, S8 and S10 of RRSV were carried out during this three years period. The results demonstrated that Pns6 is a nonstructural nucleic acid binding protein, P8 is a structural protein and a component of viral inner-capsid and P10 has ATPase activity. The viral structural protein associated with ATPase activity in genus of phytoreovirus is found for the first time.

以水稻齿裂矮缩病毒为实验对象，研究植物dsRNA病毒的由昆虫媒介传播的侵染机制。证明病毒外壳突起蛋白在病毒－昆虫－水稻传播过程中的重要作用。应用高表达病毒突起蛋白的转基因水稻，喂饲传毒昆虫后，可以抑制病毒在昆虫体内的增殖，从而抑制病毒对水稻的侵染，从而开创了转基因水稻防治病毒的一条比直接杀虫对环境有利的新途径。而且进一步阐明植物dsRNA病毒在昆虫和寄主植物间相互作用的机制。同时还对病毒的第6、第8和第10（S6，S8和S10）基因组的功能进行了研究，发现S6的产物是一个核酸结合蛋白，P8是一个病毒的结构蛋白，而S10的产物具ATP酶的活力。这是在植物dsRNA病毒中的首次报导。