中文摘要
本项目采用LbL模板和复凝聚技术，首次制备了具有pH、磁、热多重响应性聚电解质载药微囊，即以聚苯乙烯乳胶粒、二氧化硅和碳酸钙颗粒、布洛芬药物颗粒为模板，将聚电解质壳聚糖CHS、海藻酸钠ALG和羧甲基纤维素CMC、磁性纳米颗粒、带负电荷或双亲性磷脂、粘土颗粒在单分散胶体和药物微晶模板表面进行组装，得到具有多重响应性的微囊或载药微粒。pH响应性由弱聚电解质CHS和ALG提供，磁响应性由Fe3O4磁性纳米粒子在囊壁中的分布来调节，热响应性通过磷脂膜的相转变来实现。普通的聚电解质载药微囊由于通透性大而普遍存在突释现象，造成的输运过程中的药物泄露问题，本项目制备的微囊创新之一即在于在普通微囊中引入磁性纳米粒子和热敏磷脂，利用了热敏磷脂膜的致密结构缓解了聚电解质微囊的突释现象，另一方面利用Fe3O4纳米颗粒的磁导向功能和热疗功能，使得微囊既可实现磁靶向定位，又可在外交变磁场作用下使周围环境升温至磷脂膜的相转变温度，磷脂膜结构转变为疏松结构引起药物释放，从而实现了药物的可控释放。
英文摘要
A new type of drug delivery carrier with pH-, thermo- and magnetic- sensitivity have been fabricated via the layer-by-layer self-assembly and template technique. The natural polyelectrolytes of chitosan, soldium alginate and carboxymethyl cellulose (CMC), Fe3O4 nanopartcicle, polar lipid and clay nanoparticles are chosen to design the microcapsules, which obtained after decomposing the template of colloidal particles or drug crystal. Normal drug loaded polyelectrolyte multilayer microcapsules have the problem of sharp initial bursting because the high porosity and hydrophility. One of the advantages of this work is that lipid films coating on the outer surface of the microcapsules had compact structure under the phase transition temperature, which prevented the drug release and decrease the initial bursting effectively. The other advantage is the utilization of both the magnetic property and thermo-therapy function of the Fe3O4 nanoparticles. The magnetic property of Fe3O4 nanoparticles endowed the function of magnetic targeting to the microcapsules. The latter was used to adjust the temperature to the phase transition of lipid, above which drug could permeated through the microcapsules resulting in the controlled drug delivery.
结题摘要
本项目采用LbL模板和复凝聚技术，首次制备了具有pH、磁、热多重响应性聚电解质载药微囊，即以聚苯乙烯乳胶粒、二氧化硅和碳酸钙颗粒、布洛芬药物颗粒为模板，将聚电解质壳聚糖CHS、海藻酸钠ALG和羧甲基纤维素CMC、磁性纳米颗粒、带负电荷或双亲性磷脂、粘土颗粒在单分散胶体和药物微晶模板表面进行组装，得到具有多重响应性的微囊或载药微粒。pH响应性由弱聚电解质CHS和ALG提供，磁响应性由Fe3O4磁性纳米粒子在囊壁中的分布来调节，热响应性通过磷脂膜的相转变来实现。普通的聚电解质载药微囊由于通透性大而普遍存在突释现象，造成的输运过程中的药物泄露问题，本项目制备的微囊创新之一即在于在普通微囊中引入磁性纳米粒子和热敏磷脂，利用了热敏磷脂膜的致密结构缓解了聚电解质微囊的突释现象，另一方面利用Fe3O4纳米颗粒的磁导向功能和热疗功能，使得微囊既可实现磁靶向定位，又可在外交变磁场作用下使周围环境升温至磷脂膜的相转变温度，磷脂膜结构转变为疏松结构引起药物释放，从而实现了药物的可控释放。