中文摘要
雄蕊发育和雌蕊发育的精确协同是植物可育的关键因素。花丝伸长受阻、花粉成熟延迟和花药破裂异常都将导致植物雄性不育。拟南芥中,AtB31、AtB34和AtB67三个转录因子属于DELLA类蛋白抑制基因,在赤霉素和茉莉素互作调控植物雄性不育的过程中可能具有重要作用。我们拟从观察三个基因单突变体、双突变体和三突变体的表型入手,通过RT-PCR和Northern等手段,研究在外施赤霉素和茉莉素条件下,这三个基因在不同突变体(自身的单、双、三突变体,赤霉素、茉莉素生物合成和信号传导突变体等)中的表达,阐明AtB31、AtB34和AtB67在植物雄蕊发育中的功能,并解释赤霉素和茉莉素信号互作调控这三个基因表达的分子机制。.该项目的实施和完成,对了解AtB31、AtB34和AtB67三个转录因子在雄蕊发育中的功能具有重要意义,尤其是有助于探讨赤霉素和茉莉素信号互作调控植物雄性不育的分子机理。
英文摘要
Gibberellin and jasmonates are all important phytohormones, which regulate the.development and the resistance of plant. By involved in the SCF ubiquitin protein.degradation system, the two phytohormones regulate their important functional genes in.their signal chains, respectively. However, it is unclear that whether some interactive.events exist between these two phytohormones....Precise coordination between stamen and pistil development is essential to make a.fertile flower of Arabidopsis. Mutations impairing stamen filament elongation, pollen.maturation or anther dehiscence will cause male sterility. Both gibberellin and.jasmonates can regulate stamen development. Our former experiments showed that three.transcription factors are JA and GA-inducible floral specific genes that are likely.essential for stamen growth. Based on the former results, we will investigate the.interactive moleculer mechanism between gibberellin and jasmonates to modulate stamen.growth in Arabidopsis. The completion of the present project will throw light on.discovering the interactive moleculer mechanism between gibberellin and jasmonates, and.bring theoretical significance that how the phytohormone function together to control.male sterility in Arabidopsis.
结题摘要
雄蕊发育和雌蕊发育的精确协同是植物可育的关键因素。花丝伸长受阻、花粉成熟延迟和花药破裂异常都将导致植物雄性不育。拟南芥中,AtB31、AtB34和AtB67三个转录因子属于DELLA类蛋白抑制基因,在赤霉素和茉莉素互作调控植物雄性不育的过程中可能具有重要作用。我们拟从观察三个基因单突变体、双突变体和三突变体的表型入手,通过RT-PCR和Northern等手段,研究在外施赤霉素和茉莉素条件下,这三个基因在不同突变体(自身的单、双、三突变体,赤霉素、茉莉素生物合成和信号传导突变体等)中的表达,阐明AtB31、AtB34和AtB67在植物雄蕊发育中的功能,并解释赤霉素和茉莉素信号互作调控这三个基因表达的分子机制。该项目的实施和完成,对了解AtB31、AtB34和AtB67三个转录因子在雄蕊发育中的功能具有重要意义,尤其是有助于探讨赤霉素和茉莉素信号互作调控植物雄性不育的分子机理。
