中文摘要
本项目着眼于对生物活性自由基具有识别与信号传导功能的自旋标记量子点荧光探针的设计与制备，对半导体量子点、金量子点的自旋标记（顺磁修饰）技术、量子点与氮氧自由基间电子转移与光诱导还原等影响量子点发光性能的机理进行了研究，发展了自组装、配体交换等量子点自旋标记技术；发展了SiO2包覆型、金纳米壳球型等自旋标记多模式响应荧光探针；对自旋标记荧光探针应用于氨基酸、多肽、蛋白质生物活性自由基的识别与信号传导进行了初步的研究。该项目研究发展了一类水溶性好、生物相容性好、光稳定、能实现一元激发多元发射、具有识别生物活性自由基功能、能同时提供荧光检测、电子顺磁共振波谱检测信号的多模式响应分子探针，拓展了荧光探针设计的思路，丰富了量子点光物理与光化学基础应用研究成果，并通过该项研究进一步推进了荧光探针设计、分子识别与信号传导相关基础理论的研究。
英文摘要
This project has been aimed at designing and developing quantum dots fluorescence probes that are sensitive to bio-reactive radicals, and has been exploring approaches for spin-labeling of semiconductor quantum dots and gold nanoparticle (Gold quantum dots) and studying the basic theory that relates to the mechanism of spin-labeled quantum dots sensoring the paramagnetic property of targets. Non-covalent electrostatic self-assembly approach and ligand-exchange approaches have been developed for synthesizing water-soluble, off-on spin-labeled quantum dots conjugate. SiO2 shell and gold nanoshell spin-labeled fluorescence probes, with a virtue of combined fluorescence, ESR and MRI multi-model signaling, have been obtained. Efforts have been put in testing the application of spin-labeled probe for targeting bio-molecule based radicals shuc as amino acids, peptides and proteins. With both the radical trapping property of the spin-functional group and the multiple merits of quantum dots such as high quantum yield, photostability, narrow and systmatic emission band, adjustable emission wavelenth, water soluable and bio-compatibility, this kind of new quantum dots may be unique in probing bio-reactive radical, by its merits suitable for advanced technique, such as confocal fluorescence microscopy,two-photon confocal microscopy, and fluorescence correlation spectroscopy. This study is of important in understanding the reaction mechanism of functional quantum dots sencoring the bio-molecule based radical, and extending the biological application of quantum dots.
结题摘要
本项目着眼于对生物活性自由基具有识别与信号传导功能的自旋标记量子点荧光探针的设计与制备，对半导体量子点、金量子点的自旋标记（顺磁修饰）技术、量子点与氮氧自由基间电子转移与光诱导还原等影响量子点发光性能的机理进行了研究，发展了自组装、配体交换等量子点自旋标记技术；发展了SiO2包覆型、金纳米壳球型等自旋标记多模式响应荧光探针；对自旋标记荧光探针应用于氨基酸、多肽、蛋白质生物活性自由基的识别与信号传导进行了初步的研究。该项目研究发展了一类水溶性好、生物相容性好、光稳定、能实现一元激发多元发射、具有识别生物活性自由基功能、能同时提供荧光检测、电子顺磁共振波谱检测信号的多模式响应分子探针，拓展了荧光探针设计的思路，丰富了量子点光物理与光化学基础应用研究成果，并通过该项研究进一步推进了荧光探针设计、分子识别与信号传导相关基础理论的研究。