研究肾虚型自然流产患者膜孕激素受体(PR)表达.建立妊娠期肾虚和黄体抑制病证结合模型,研究证候与病理造模对子宫蜕膜PR基因表达的影响,以补肾安胎代表方寿胎九,首选取中药菟以及西药黄体酮对组织PR基因表达的调控,对胚胎组织细胞调亡的影响。为阐明:肾为先天之本"提出新的依据.

Spontaneous abortion is a frequent disease and has the tendency of recrudescence . It impairs the somatopsychic health of women . The main cause of spontaneous abortion is the maladjustment of incretion, especially luteal phase defect ( Which divided into two kinds, one is the secretion deficiency of progesterone （P）, the other is the insufficiency of progesterone receptor（PR） of endometrium with normal progesterone level). The patients with luteal phase defect often manifest the kidney deficiency syndrome in clinic. The Chinese herbs with the functions of invigorating kidney and miscarriage prevention can prevent and cure spontaneous abortion in clinical research, which suggests the reinforcing kidney herbs can improve the functions of luteum. But the mechanisms are not clear. 1 Through studying the gene expression levels of PR and transforming growth factorβ1 in spontaneous abortion patients with the syndrome of kidney-deficiency, we demonstrated the relativity between the syndrome of kidney-deficiency and pathological mechanisms of luteal phase defect in gestation. 2 We established model which integrating kidney-deficiency syndrome and luteal phase defect to simulate the frequent model of spontaneous abortion in clinic, and investigated the gene expression levels of PR of deciduas of spontaneous abortio

自然流产在妇产科颇为常见，有反复发生的倾向，对妇女的心身健康具有较大的影响。其中以内分泌失调，尤其是黄体功能不全（又分为孕激素（P）分泌不足及P水平正常而子宫内膜的孕激素受体（PR）含量不足两种类型）较为多见。临床上黄体功能不全者常表现为肾虚证候。在临床研究中，补肾安胎中药对自然流产具有确切的防治作用。并提示补肾中药能改善黄体功能，但其作用机理尚未明确。本项目主要研究了：（1）肾虚型自然流产患者子宫蜕膜PR和转化生长因子β1的基因表达水平，论证妊娠期肾虚证候和黄体功能不全病理机制的相关性。（2）建立妊娠期肾虚和黄体抑制病证结合模型，模拟临床上较常见的自然流产类型，研究肾虚和黄体抑制对妊娠子宫蜕膜PR基因表达的影响。（3）以补肾安胎代表方寿胎丸、首选中药菟丝子为施加因素，比较补肾中药复方、单味以及西药黄体酮对靶组织PR基因表达的调控，对胚胎组织细胞凋亡的影响。论证了补肾安胎治法主要通过对靶组织PR 基因表达的上调作用，增强孕酮的效应，从而达到保胎之目的，阐明中药安胎的作用基础，也为阐明"肾为先天之本"提出新的依据。研究成果有助明确中医学补肾安胎治法的药理机制，促进中医药的开发并走向世界