中文摘要
从发病禽群中分离新城疫病毒（NDV）246株，建立了NDV病毒库，病毒库中现有不同时间、地区、宿主来源的病毒418株。测定所有分离株F和HN基因序列，并确定了分离株的基因型，同时测定了43个代表株的全基因组序列，结合GenBank中公布序列建立了102株NDV全基因及4000多条6种单个基因的序列数据库。序列分析显示：在NDV进化中，各基因呈同步进化；鹅群中强毒株基本上属于基因VIId亚型与流行株一致，且鸡与鹅源VIId亚型毒株无明显区别。与其它基因型相比，VIId亚型NDV出现了多个特征性的氨基酸替换。致病性试验结果显示：ND强毒的致病性与基因型密切相关；与早期毒株相比，VIId亚型毒株可导致脾脏和肠道淋巴组织的严重损伤和坏死，因此，VIId亚型NDV对鹅免疫系统侵嗜能力的增强是导致其发病的重要原因。根据受体结合位点与VIId亚型主要蛋白特征性氨基酸鉴定结果，利用反向遗传技术，以ZJ1为骨架构建了嵌合表达鸡源IV型Herts/33株P、F和HN基因的重组病毒，重组病毒致病性试验结果表明，HN在NDV种间传播中扮演了重要角色。此外，研制出了可预防VIId亚型NDV感染的新疫苗。
英文摘要
Two hundred and forty-six Newcastle disease viruses (NDVs) were isolated from the diseased poultry flocks in recent years. These viruses together with the previously collected strains constituted a virus depository which contains 418 NDVs representing all genotypes with different host, geographical and chronological origins. F and HN gene sequences of all isolates and 43 complete genome sequences of representative strains have been determined. Base on the NDV sequences derived in this study and published sequences in GenBank, a gene sequence database, including 102 complete genomes and more than 4000 individual genes, was set up. Comprehensive analysis of the gene sequences showed that six individual genes of NDV have evolved synchronously and that almost all of the goose-origin NDVs belong to subgenotype VIId which is the dominant genotype prevalent in Asia since 1990s. Compared with other genotypes, several characteristic amino acids in each of different proteins of VIId viruses have been identified. However, no distinction is detected between the goose- and chicken-origin VIId NDVs.In the animal infection experiments, the pathogenicity and virulence of the viruses are closely related with their genotypes. Notably, genotype VIId NDVs induce much more severe necrosis of spleen and multi-ulcer of intestinal ly
结题摘要
从发病禽群中分离新城疫病毒（NDV）246株，建立了NDV病毒库，病毒库中现有不同时间、地区、宿主来源的病毒418株。测定所有分离株F和HN基因序列，并确定了分离株的基因型，同时测定了43个代表株的全基因组序列，结合GenBank中公布序列建立了102株NDV全基因及4000多条6种单个基因的序列数据库。序列分析显示：在NDV进化中，各基因呈同步进化；鹅群中强毒株基本上属于基因VIId亚型与流行株一致，且鸡与鹅源VIId亚型毒株无明显区别。与其它基因型相比，VIId亚型NDV出现了多个特征性的氨基酸替换。致病性试验结果显示：ND强毒的致病性与基因型密切相关；与早期毒株相比，VIId亚型毒株可导致脾脏和肠道淋巴组织的严重损伤和坏死，因此，VIId亚型NDV对鹅免疫系统侵嗜能力的增强是导致其发病的重要原因。根据受体结合位点与VIId亚型主要蛋白特征性氨基酸鉴定结果，利用反向遗传技术，以ZJ1为骨架构建了嵌合表达鸡源IV型Herts/33株P、F和HN基因的重组病毒，重组病毒致病性试验结果表明，HN在NDV种间传播中扮演了重要角色。此外，研制出了可预防VIId亚型NDV感染的新疫苗。