中文摘要
显齿蛇葡萄干叶中黄酮的质量分数达45.52%,仅二氢杨梅素的含量就达39.50%,被誉为"植物黄酮之王"; 不同于其他富含黄酮植物倾向于合成黄酮苷,该植物以苷元为主。然而,目前显齿蛇葡萄合成黄酮苷元的机制尚不明确,因此,开展显齿蛇葡萄次生代谢积累机制研究具有重要的理论和实际意义。本研究采用HPLC等仪器分析手段测定显齿蛇葡萄中二氢杨梅素及其生源相关化合物的含量,并借助SPSS软件的相关性分析法探求化合物含量之间的相关性,明确二氢杨梅素代谢积累的关键前体及竞争性底物;研究极端二氢杨梅素含量植株在同一生境栽培后二氢杨梅素及其生源相关化合物含量的动态变化规律,并借助DPS软件的灰色关联度法分析气候因子与二氢杨梅素之间的相关性,阐明二氢杨梅素代谢积累生理生态机制。以期为进一步研究二氢杨梅素代谢积累的分子机制奠定基础,也为其他富含黄酮苷元植物的代谢研究提供理论依据。
英文摘要
The content of total flavonoids and dihydromyricetin in dried leaves of Ampelopsis grossedentata is so high, accounting for 45.52% and 39.50%, respectively,which is honored as "King of Flavonoids-rich plant". Ampelopsis grossedentata synthetizing flavone aglycones as its main secondary metabolites, which differs from other flavonoids-rich plants yielding flavonoid glycosides. At present, the mechanism of synthetizing flavone aglycones of Ampelopsis grossedentata is still unclear. Therefore, it is of great theoretic and practical significance to study the accumulation of secondary metabolites of the plant. In order to illuminate the key precursors and competitive substrates involved in the biosynthetic pathway of dihydromyricetin, the content of dihydromyricetin and related compound in Ampelopsis grossedentata will be detected by analytical instruments such as high-performance liquid chromatography. With the aid of SPSS software and correlation analytic methods, the correlation between the content of compounds will be analyzed. The correlation between climatic factors and the content of compounds will be analyzed using Data Processing System (DPS) and grey correlation degree analytic method. In order to clarify the physiological and ecological mechanism of the metabolic accumulation of dihydromyricetin, the dynamic changes of dihydromyricetin and the related compounds from dihydromyricetin-rich and dihydromyricetin-poor plants cultivated in the same habitat will be studied. The results will lay a foundation for further study on the molecular mechanism of the metabolic accumulation of dihydromyricetin, and provide theoretical basis for studies on the metabolism of other flavone-rich plants.
