黄曲霉毒素（AFT）是普遍污染食品和饲料的强毒性和致癌性物质，严重威胁人畜健康。本项目前期研究发现，低浓度肉桂醛可以调控AFT生物合成而抑制毒素产生，但关键调控基因及其调控路径不清楚。在此基础上，本项目拟采用RNA-seq、二维电泳和iTRAQ定量蛋白质组学等技术，分析肉桂醛处理下黄曲霉RNA和蛋白的差异表达，筛选参与肉桂醛抑制AFT生物合成的候选调控基因；构建调控基因敲除突变体，分析候选调控基因对AFT生物合成基因簇内关键调控基因（aflR和aflS）和合成基因表达以及AFT产量的影响，确定关键调控基因及其功能；通过酵母双杂交技术鉴定调控基因与AFT生物合成关键基因的相互作用，揭示调控基因对AFT合成基因的调控机制，明确肉桂醛抑制AFT生物合成的基因调控路径。上述研究将初步阐明肉桂醛阻断和抑制AFT生物合成的分子调控机理，为利用肉桂醛防控AFT污染食品和饲料提供理论基础。

Aflatoxins (AFT) are carcinogenic, genotoxic and immunotoxic. Foods and feeds are generally contaminated with AFT, which become a great health threaten to humans and animals. In our previous study, we found that at lower concentration, the reduction of AFT production by cinnamaldehyde was due to down-regulation of AFT biosynthesis. But the key regulatory genes and their regulatory pathways of action were unclear. In the present study, RNA-seq, two-dimensional electrophoresis as well as iTRAQ labeling techniques will be used to analyze the difference at RNA and protein levels in Aspergillus flavus with cinnamaldehyde or without. Then the candidate regulatory genes will be selected. Then the mutants of the regulatory genes knocked out will be constructed. The effect of regulator genes on the expression of AFT biosynthetic regulatory genes (aflR and aflS) and structural genes and AFT production will be analyzed. The key regulatory genes and their functions will be determined. The interactions of regulatory genes and AFT biosynthetic genes will be identified by yeast two-hybrid assay, and the regulatory mechanism of the regulatory genes on AFT biosynthetic genes will be elucidated. The gene regulatory pathway of inhibition of AFT biosynthesis by cinnamaldehyde will be constructed. This research can preliminarily reveal the regulatory mechanism of inhibition of AFT biosynthesis by cinnamaldehyde. And this research is also significant for usage of cinnamaldehyde to control AFT contamination in foods and feeds.